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. 2001 Aug;126(4):1738–1753. doi: 10.1104/pp.126.4.1738

Figure 5.

Figure 5

Nuclear localization of NtMYBAS1-sGFP fusion proteins. Tobacco pollen grains bombarded with pLAT52-sGFP control plasmid (a) and pLAT52-sGFPM1-3 plasmids harboring NtMYBAS1-sGFP fusion constructs (b–d). a, The control, sGFP alone, remains predominantly in the cytoplasm. b, pLAT52-sGFPM1. N-terminal 126 amino acids of NtMybAS1 result in localization of sGFP exclusively to the pollen nucleus. c, pLAT52-sGFPM2. N-terminal 119 amino acids of NtMybAS1 result in a dramatic reduction in nuclear localization. d, pLAT52-sGFPM3. The MTRLKRRQRA motif alone is not sufficient for exclusive nuclear targeting of sGFP. R2R3, MYB repeats; TEV-L, tobacco etch virus 5′-untranslated region; C3′, cauliflower mosaic virus 35S transcript polyadenylation sequence; n, NcoI; B, BspHI; NB, NcoI/BsphI junction.