Figure 9.

Trans-activation of tobacco promoters by NtMYBAS1. a, Effector and reporter constructs used in cotransfection experiments. The 35S::NtMYBAS1 effector plasmid consisted of the coding region of NtmybAS1 under control of the CaMV 35S promoter. The reporter plasmids consist of two tobacco PAL promoters, PALA (Pellegrini et al., 1994) and gPAL1 (Tomoko et al., 1996); tobacco polygalacturonase NPG1 promoter (Tebbutt et al., 1994) and tobacco neIF4A8 promoter (Brander and Kuhlemeier, 1995) fused to the GUS reporter gene. b, Response of reporter constructs to NtMYBAS1 in transient expression assays. Tobacco leaf protoplasts were cotransfected with each reporter plasmid together with 35S::NtMYBAS2. Error bars represent se (n > 7).