Table 1. p53 response elements and oligonucleotides used in the study.
| Name | Abbreviation | Sequencea | Remarks | Reference |
|---|---|---|---|---|
| Consensus | CON | 5′-bio-(N)20-AGGCATGTCT AGGCATGTCT-3′ | 100% consensus match | 11 |
| Mutant | MUT | 5′-bio-(N)20-AGGa c g tTCT AGGa c g tTCT-3′ | Non-binding mutant control | |
| p21 | p21 | 5′-bio-(N)20-GAACATGTCC cAACATGTT g-3′ | Cyclin-dependent kinase inhibitor | 3 |
| GADD45 | G45 | 5′-bio-(N)20-GAACATGTCT AAGCATGCTg-3′ | Cell cycle regulator, involved in DNA repair | 5 |
| GADD3T | G3T | 5′-bio-(N)20-GAACATGTCT AAGCATGTTg-3′ | Modified GADD45 | |
| GADD3C | G3C | 5′-bio-(N)20-GAACATGCCT AAGCATGCTg-3′ | Modified GADD45 | |
| GADD4T | G4T | 5′-bio-(N)20-GAACATGTCT AAACATGTTg-3′ | Modified GADD45 | |
| GADD4C | G4C | 5′-bio-(N)20-GAGCATGCCT AAGCATGCTg-3′ | Modified GADD45 | |
| BAX | BAX | 5′-bio-(N)20 -t cACAAGTTag AGACAAGCCTggg cgt GGGCTAtaTT-3′ | Apoptosis promoting | 7 |
aOnly the biotinylated strand is shown; consensus-fitting bases are in upper case and residues 3 and 8 are in bold.