Table 1.

Antibodies used in the study.

Antibody	M/P	Clone	Source	Specificity	Significance	Dilution
ER	M	1D5	DAKO	Semiquantitative ER nuclear detection in BC	+—≥1%	1:50
PR	M	PgR636	DAKO	Semiquantitative PR nuclear detection in BC.	+—≥20%	1:50
HER2	M	4B5	DAKO	Membrane expression HER2 in BC	+—>10%	1:500
Ki-67	M	MIB-1	DAKO	Nuclear protein that is associated with and may be necessary for cellular proliferation Cellular marker for proliferation (Ki-67 Index)	Low—<14%	1:10
					Moderate—<20%
					High—>20%

Legend: BC = Breast cancer; ER = Estrogen Receptor; HER-2 = Receptor tyrosine kinase erbB-2; Ki-67 = Antigen Kiel 67; PR = Progesterone Receptor; M = Monoclonal; P = Polyclonal.