Table 5. Southern hybridization analysis of H.pylori hpyV and hpyIVA R-M systems.

H.pylori methyltransferase	RE	Strain designation	Genotype (PCR)		Phenotypec	Southern hybridizationd
			R-Ma	Mb
hpyV	TaqI	26695	+e	+f	R	+
		J99	+	+	R	+
		96-27	Truncatedf	+	R	+
		00-205	+	+	S	+
		00-336	None	None	S	–
		00-165	None	Truncated	S	–
hpyIVA	HinfI	26695	+	+	R	+
		J99	+	+	R	+
		97-701	+	+	S	+
		99-33	No	Truncated	S	–
		97-789	+	+	R	+
		97-32	Empty	+	R	+

^aBased on primers HP0259F/HP0262R for hpyV; F3/R3 for hpyAIVRM.

^bBased on primers HP0260/HP0261 for hpyVM; F2/R2 for hpyAIVM.

^cPhenotype refers to resistance (R) or susceptibility (S) to digestion of chromosomal DNA by the indicated RE.

^dProbe is the PCR product amplified by primers HP0260/HP0260R for hpyVM (1074 bp) and primers F2/R2 for hpyAIVM (914 bp). +, hybridizing fragment of the specified size; –, no fragment was identified.

^e+, expected size (1889 bp for hpyVRM and 2524 bp for hpyAIVRM).

^fThe truncated product was ∼400 bp.

^g+, expected size (1156 bp for hpyVM and 914 bp for hpyAIVM).