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. 2002 Jun 1;30(11):2349–2357. doi: 10.1093/nar/30.11.2349

Figure 5.

Figure 5

The chromatin-associated hOGG1–EGFP is phosphorylated on a serine residue in vivo. (A) HeLa cells expressing the hOGG1–EGFP fusion protein were extracted to obtain the cytoplasmic fraction, the whole chromatin fraction, a high salt wash and the core nuclear matrix. Proteins from equal cell equivalents of each fraction were analysed by western blotting with the indicated antibodies. (B) Equal cell equivalents from each fraction (lanes 1–8) or 10 ng of hOGG1 (lanes 9 and 10) were mock treated (lanes 1, 3, 5, 7 and 9) or treated with λ-PPase (lanes 2, 4, 6, 8 and 10) before measuring hOGG1 activity in the presence of HAP1 endonuclease as described in Figure 4. 13* nt, 13 mer with a 3′-OH produced by the addition of HAP1 after hOGG1-strand nicking activity.