Figure 7.

Effects of ATF3ΔZip2 on transcriptional repression by ATF3. (A) Cos-7 cells were transfected with pME (lane 1), pME-Flag-ATF3 (lane 2) or pME-Flag-ATF3ΔZip2 (lane 3) and their whole-cell extract were analyzed by western blot using monoclonal anti-Flag antibody. (B) Cos-7 cells were co-transfected with pLuc-4xATF and increasing amounts of pME-Flag-ATF3 or pME-Flag-ATF3ΔZip2. At 24 h post-transfection, cell extracts were assayed for luciferase activity as detailed in the Materials and Methods. Results represent mean ± SE from three independent experiments. Statistically significant inhibition or activation are indicated (*P < 0.05; **P < 0.005).