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. 2002 Jun 1;30(11):2555–2564. doi: 10.1093/nar/30.11.2555

Figure 1.

Figure 1

RP-HPLC chromatogram of dGuo reacted with HOCl with (A) or without (B) termination by N-AcCys. dGuo (1 mM) was incubated with 1 mM NaOCl in 100 mM sodium phosphate buffer (pH 7.4) at 37°C for 15 min. The samples with or without further addition of 2 mM N-AcCys were separated on an ODS column (4.6 × 250 mm). The eluent was 20 mM sodium phosphate buffer (pH 7.0) containing acetonitrile. The acetonitrile concentration was increased from 0 to 15% over 20 min in linear gradient mode. The column temperature was 30°C and the flow rate 1 ml/min. (Inset) On-line UV spectrum of 1.