Figure 11.

pH dependence of the yields of dDiz (closed circles), dIz (open triangles) and 8-Cl-dGuo (open squares) on the dGuo/HOCl system in the absence (A) and presence (B) of NaCl. dGuo (1 mM) was incubated with 1 mM NaOCl in 100 mM sodium phosphate buffer of various pH at 37°C for 15 min in the absence and presence of NaCl (100 mM) and subsequently incubated with 2 mM N-AcCys. The concentration of the products was quantified by RP-HPLC analysis. The data points are expressed as means ± SD (n = 3).