Figure 7.

RP-HPLC analyses for a precursor of dIz. (A) RP-HPLC chromatogram of purified 3 (dIz-Cl) from a reaction mixture of dGuo with HOCl without final N-AcCys treatment. Product 3 was isolated by RP-HPLC from the reaction mixture of dGuo (1 mM) incubated with 1 mM NaOCl in 100 mM sodium phosphate buffer (pH 7.4) at 37°C for 15 min. (Inset) On-line UV spectrum of 3. (B) RP-HPLC chromatogram of a solution of purified 3 incubated with N-AcCys. The isolated 3 was incubated with 0.5 mM N-AcCys in 100 mM sodium phosphate buffer (pH 7.4) at 37°C for 5 min and the reaction mixture was injected onto a RP-HPLC column. RP-HPLC conditions were as described inFigure 1.