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Expression of Purγ-A and Purγ-B mRNAs in human testis. Human Purγ-A or Purγ-B mRNA in a human testis mRNA preparation (Clontech) was reverse transcribed with either primer HPCA3 or HPCB3 and was then PCR amplified with either primers HPCA3 and HPC5 or primers HPCB3 and HPC5. For negative controls there was no incubation time in the reverse transcription step.
