Figure 6.

Failure of U1antiGFPsaph to inhibit pOB₄GFPtpz. The same U1antiGFPsaph constructs that inhibited pOB₄GFPsaph expression in Figure 5C and D were co-transfected with cells expressing pOB₄GFPtpz and selected for 4 weeks. (A). Fluorescence microscopy (×100) of representative clones: (1) pOB₄GFPtpz transfected with U1 snRNA; (2) pOB₄GFPtpz transfected with U1antiGFPsaph433, which has a 1 bp mismatch in the target domain; (3) pOB₄GFPtpz transfected with U1antiGFPsaph196, which has a 2 bp mismatch in the target domain. (B) Flow cytometry of the pooled colonies described in (A). M1 and M2 represent the domains in GFP-negative and GFP-positive cells, respectively. 10 000 cells were counted and their intensity is plotted in RFU.