Table 3. Quantitation of flow cytometry showing inhibition of pOB4GFPtpz by U1antiGFPtpz in three selected clones (see Fig. 2A).
Treatment | Percent of total cell population | Mean strength | Fluorescence index (×103) | ||
---|---|---|---|---|---|
M1 | M2 | M1 | M2 | ||
pOB4GFPtpz | 54.7 ± 16.3 | 45.4 ± 1.3 | 5.5 ± 0.4 | 275.1 ± 190.0 | 12.5 ± 0.2 |
pOB4GFPtpz + U1 snRNA | 57.5 ± 19.8 | 50.0 ± 19.9 | 5.0 ± 0.9 | 897.6 ± 411.0 | 44.9 ± 8.2 |
pOB4GFPtpz + U1antiGFPtpz196 | 98.0 ± 0.1a | 4.3 ± 0.1a | 3.0 ± 0.2 | 97.3 ± 38.3a | 0.4a |
See Table 2 for details. Data is presented as means ± SD.
aP < 0.05 compared with either pOB4GFPtpz alone or pOB4GFPtpz and U1 snRNA.