Table 4. Quantitation of flow cytometry showing inhibition of pOB4GFPtpz by three different U1antiGFPtpz constructs in pooled clones.
| Treatment | Percent of total cell population | Mean strength | Fluorescence index (×103) | ||
|---|---|---|---|---|---|
| M1 | M2 | M1 | M2 | ||
| No transfection | 99.9 | 0.1 | 2.5 | 11.6 | 0 |
| pOB4GFPtpz | 10.9 | 89.4 | 5.1 | 782.9 | 70.0 |
| pOB4GFPtpz + U1 snRNA | 8.8 | 91.6 | 5.7 | 244.5 | 22.4 |
| pOB4GFPtpz + U1antiGFPtpz433 | 31.6 | 66.4 | 6.1 | 45.0 | 2.9 |
| pOB4GFPtpz + U1antiGFPtpz196 | 32.3 | 69.1 | 5.7 | 59.1 | 4.0 |
| pOB4GFPtpz + U1antiGFPtpz236 | 32.4 | 68.7 | 5.8 | 48.1 | 3.3 |
See Table 2 for an explanation of this table.