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. 2005 Jul 1;19(13):1512–1517. doi: 10.1101/gad.1302205

Figure 4.

Figure 4.

The hTREX complex is recruited to spliced mRNAs by a transcription-independent mechanism. (A) T7 AdML pre-mRNA was incubated under splicing conditions for 90 min, and aliquots (5 μL) of the reaction were used for immunoprecipitations. (B) T7-AdML pre-mRNA (lanes 1–5) or corresponding cDNA transcript (lanes 6–10) was incubated under splicing conditions, followed by immunoprecipitations. The percentage immunoprecipitated relative to input was quantitated by PhosphorImager. One-fourth of the input was loaded on the gel. (C–E) Ftz pre-mRNA was synthesized and spliced in an RNAP II transcription/splicing system in HeLa nuclear extract for the times indicated (input). An aliquot (5 μL) from each time point was used for immunoprecipitations. One-fourth (C) or one-tenth (D,E) of the input was loaded on the gel. (*) Nonspecific band. U6 snRNA is labeled during incubation of the RNAP II transcription/splicing system. Control antibodies (cntrl a–c) were rabbit antibodies to unrelated proteins.