Figure 7.

SCON-2 is rapidly degraded in the csn-2 mutant, resulting in its drastically reduced expression level. (A) Expression level of Myc-SCON-2 in the wild-type and csn-2^KO strains in LL. Two independent transformants of each strain were used. (B) Western blot analysis showing the degradation of Myc-SCON-2 in the wild-type and csn-2^KO strains. (C) Race tube assays showing the complementation of the circadian conidiation rhythm defect of the cns-2^KO strain by the expression Myc-His-CSN-2. (D) Silver-stained SDS-PAGE gel showing biochemical purification of the Neurospora CSN. A wild-type strain was used as the negative control. CSN subunits identified by mass spectrometry analysis are indicated. Asterisks indicate the two IgG bands.