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. 2024 Dec 30;26(1):216. doi: 10.3390/ijms26010216

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© 2024 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Multiple alignment and phylogenetic tree of ManEM6. (a) Multiple sequence alignment with glycoside hydrolase family 26 (GH26): β-endo-mannanase showed three putative catalytic residues (Glu189, Asp254, and Glu296), which are shown in dark-shaded letters. Asterisks (*) indicate consensus amino acids. Each protein sequence used for alignment was collected from GenBank, including Bacillus sp. JAMB750 [29], Bacillus subtilis [31], Cellvibrio japonicas [32], Bacteroides ovatus [33], Bacteroides sp. 2_1_33B [34]. (b) The phylogenetic tree of ManEM6 and other closely related enzymes was reconstructed using the neighbor-joining method (MEGA5.1 software). The protein sequences of related enzymes were retrieved from NCBI GenBank. Bootstrap values (>50%) at the nodes were based on 1000 replicates of the dataset. Solid circles indicate that the corresponding branches were also recovered in both the maximum parsimony and maximum likelihood trees. Bar, 0.2 substitutions per amino acid position.

Multiple alignment and phylogenetic tree of ManEM6. (a) Multiple sequence alignment with glycoside hydrolase family 26 (GH26): β-endo-mannanase showed three putative catalytic residues (Glu189, Asp254, and Glu296), which are shown in dark-shaded letters. Asterisks (*) indicate consensus amino acids. Each protein sequence used for alignment was collected from GenBank, including Bacillus sp. JAMB750 [29], Bacillus subtilis [31], Cellvibrio japonicas [32], Bacteroides ovatus [33], Bacteroides sp. 2_1_33B [34]. (b) The phylogenetic tree of ManEM6 and other closely related enzymes was reconstructed using the neighbor-joining method (MEGA5.1 software). The protein sequences of related enzymes were retrieved from NCBI GenBank. Bootstrap values (>50%) at the nodes were based on 1000 replicates of the dataset. Solid circles indicate that the corresponding branches were also recovered in both the maximum parsimony and maximum likelihood trees. Bar, 0.2 substitutions per amino acid position.