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. 2024 Dec 30;26(1):216. doi: 10.3390/ijms26010216

Figure 2.

Figure 2

Overexpression and purification of rManEM6. ManEM6 without putative N-terminal signal peptide sequence was cloned into plasmid pET21a (+) expression vector and expressed in E. coli BL21(DE3). SDS–PAGE analysis shows a homogeneous 43 kDa protein. Lanes: M, molecular weight marker; Lane 1, total cellular protein from uninduced cells; Lane 2, induced total cellular protein; Lane 3, induced soluble fraction; Lane 4, purified rManEM6 protein; Lane 5, zymogram of rManEM6 on polyacrylamide gel with 0.2% LBG (locust bean gum) and stained with Congo-red.