Figure 2.

Binding of recombinant GKLF to the bcn-1 motif in vitro. (A) The sequence of double-stranded synthetic oligonuleotide containing either wild-type or mutated bcn-1 element that was used as a competitor in the binding reaction. (B) Autoradiograph of the gel from EMSA. DNA-binding reaction was carried out in 10 µl binding buffer containing recombinant rat GST–GKLF and ³²P-labeled double-stranded oligonucleotide containing wild-type bcn-1 element in the presence of either no competitor (none, lane 1), or 500-fold molar excess of double-stranded oligonucleotide containing either wild-type or mutated bcn-1 sequences as shown in (A). (C) Graph of the densitometric measurements of the shifted bands (B) expressed in digital light units (DLU).