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. 2002 Jun 1;30(11):2270–2279. doi: 10.1093/nar/30.11.2270

Figure 3.

Figure 3

GKLF-mediated activation of LAMC1 promoter in rat mesangial and human HeLa cells. Subconfluent rat mesangial and human HeLa cells were transiently transfected with firefly luciferase gene driven by the rat –1077/–20 LAMC1 promoter fragment with either pMt3 expression vector [(–) GKLF] or expression vector containing GKLF cDNA, pMt3-GKLF [(+) GKLF]. Renilla luciferase plasmid was used as a control for transfection efficiency. Forty-eight hours following transfection, cells were harvested and firefly and renilla luciferase activities were measured. Data are shown as mean ± SD of the ratios of firefly to renilla luciferase activities (n = 4).