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. 2002 Jun 1;30(11):2270–2279. doi: 10.1093/nar/30.11.2270

Figure 4.

Figure 4

The bcn-1 element is not required for the GKLF-mediated activation of the LAMC1 promoter in human HeLa cells. (A) Diagram of firefly luciferase reporter gene constructs that were used in transfection of HeLa cells. The sequences of the wild-type (wt-bcn-1) and mutated (mt-bcn-1-M4) bcn-1 located at –495 to –479 within the LAMC1 promoter are shown. (B) Firefly luciferase gene driven by LAMC1 promoter containing either wild-type (wt-bcn-1) or mutated (mt-bcn-1-M4) bcn-1 element fragments was co-transfected in HeLa cells with either pMt3 expression vector [(–) GKLF] or expression vector containing GKLF cDNA, pMt3-GKLF [(+) GKLF]. The renilla luciferase plasmid was used as a control for transfection efficiency. Forty-eight hours following transfection, cells were harvested and firefly and renilla luciferase activities were measured. Data are shown as mean ± SD of the ratios of firefly to renilla luciferase activities (n = 6 ).