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. 2002 Jun 1;30(11):2270–2279. doi: 10.1093/nar/30.11.2270

Figure 5.

Figure 5

Synergistic activation of rat LAMC1 promoter by GKLF and Sp1. The Drosophila SL2 cells were transiently co-transfected with rat –1077/–20 LAMC1 promoter-luciferase reporter plasmid with given amounts (ng) of either pPac-GKLF (GKLF), pPac-Sp1 (Sp1) or both expression plasmids. The expression plasmid, pPac-O, containing no insert, was used to adjust the total amount of DNA. Renilla luciferase reporter gene was also included to correct for transfection efficiency. Forty-eight hours following transfections, cells were harvested and luciferase activities in cell lysates were measured. (A) Cells transfected with increasing amounts (0, 50, 100, 1000 ng) of either pPac-GKLF (open columns) or pPac-Sp1 (closed columns). (B) Cells transfected with either pPac-O alone, pPac-GKLF (50 ng), pPac-Sp1 (50 ng) or pPac-Sp1 (50 ng) plus increasing amounts of pPac-GKLF (50–1000 ng). Data are shown as mean ± SD of the ratios of firefly to renilla luciferase activities (n = 4).