Figure 8.

The GKLF activation and zinc finger domains are required for the synergistic activation of LAMC1 promoter in SL2 cells. (A) Diagram of rat GKLF. Putative activation domain (AD), nuclear localization signal (NLS) and the three zinc fingers (Zn) are shown. (B) pPac-Sp1 (50 ng) and a series of pPac-GKLF mutants were co-transfected with the LAMC1 promoter-firefly and renilla luciferase reporter gene constructs. Activity of the LAMC1 promoter upstream of firefly luciferase was assessed in transient co-transfection experiments in SL2 cells as before. Results are shown as the mean ratio of firefly to renilla luciferase activity ± SD (n = 4).