Table 1. Variation of microarray sensitivity and specificity with oligo length: M/MM ratios for oligos corresponding to the different genes and oligonucleotide lengths.
| Length | M/MM | % M/MM >2 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| U2AF65 | Srp20 | U2AF35 | TIA1 | SXL | Average | Median | Average | Median | |
| 25mer |
3.3 ± 0.6 |
11.1 ± 1.3 |
3.6 ± 0.1 |
4.6 ± 0.1 |
4.4 ± 0.4 |
5.4 |
4.4 |
77% |
75% |
| 30mer |
2.7 ± 0.6 |
9.7 ± 0.4 |
4.3 ± 0.2 |
2.6 ± 0.4 |
2.2 ± 0.2 |
4.3 |
2.7 |
79% |
85% |
| 35mer | 1.8 ± 0.2 | 8.2 ± 0.4 | 2.8 ± 0.2 | 1.8 ± 0.4 | 1.7 ± 0.1 | 3.3 | 1.8 | 74% | 60% |
Average and median M/MM values, as well as percentage of oligos with a M/MM discrimination >2, are indicated.