Table 10. Performance of 60-nt oligos compared with 25 and 30mers: average and median values of the ratios between fluorescent signals associated with oligos of different lengths, after hybridization of labeled RNAs either generated by in vitro transcription (IVT) or isolated from HeLa cells.
| 60/25 | 60/30 | |||
|---|---|---|---|---|
| Average | Median | Average | Median | |
| IVT | 10 ± 6.7 | 10.5 | 1.8 ± 1.2 | 1.3 |
| HeLa | 7.1 ± 3.3 | 7.1 | 2.7 ± 1.4 | 2.2 |