Table 4. Variation of microarray sensitivity and specificity with the concentration of oligos spotted: fluorescent intensities for oligos of the indicated lengths, spotted at the concentrations indicated.
Oligo concentration (pmol/µl) | Signal intensities (×106) | |||
---|---|---|---|---|
25mer | 30mer | 35mer | Average | |
50 |
1.75 ± 0.07 |
2.49 ± 0.07 |
2.98 ± 0.08 |
2.41 ± 0.62 |
30 |
1.75 ± 0.05 |
2.38 ± 0.01 |
2.51 ± 0.05 |
2.21 ± 0.41 |
20 | 1.06 ± 0.09 | 2.09 ± 0.02 | 2.36 ± 0.07 | 1.84 ± 0.69 |
Average and standard deviation values for all lengths are shown.