Fig. 4.

APOBEC3F and -G inhibit Ty1 retrotransposition. (A) A schematic depicting retrotransposition by Ty1 or TyHRT yielding histidine prototrophy or luciferase activity. GA, PR, IN, RT, and LTR represent gag, protease, integrase, reverse transcriptase, and long-terminal repeat, respectively. (B and D) APOBEC3F or -G expression diminishes Ty1 or TyHRT retrotranspostion as monitored by the number of His⁺ colonies. For each condition, at least eight independent cultures were analyzed, and the error bars depict one standard error of the mean. (C and E) APOBEC3F or -G expression diminishes Ty1 or TyHRT retrotranspostion as monitored by luciferase activity. The conditions were identical to those described above.