Figure 7.

IRS-1 protein is reduced in failing human hearts. (A) Total tissue lysates from indicated samples were analyzed for Akt kinase activity with GSK3α/β as substrate. (B) Total tissue lysates from either failing hearts (n = 5) or control donor hearts (n = 4) were immunoblotted with anti–IRS-1 antibody and subsequently with anti–phospho-Akt substrate antibody. In the lower panel, densitometric quantitations for IRS-1 level and p-Akt substrate level are shown. *P < 0.005 versus control. DCM, dilated cardiomyopathy.