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. 2005 Jul 7;115(8):2244–2256. doi: 10.1172/JCI24130

Figure 1.

Figure 1

OLR1 is induced by PPARγ ligands in 3T3 L1 adipocytes. (A) Comparison of OLR1 and aP2 mRNA levels in 3T3-L1 preadipocytes (Pread), adipocytes treated with vehicle (DMSO), and rosiglitazone-treated adipocytes (Rosi). (B) Quantitation of OLR1 compared with CD36 mRNA induction by rosiglitazone, using real-time PCR. Results are normalized to mRNA levels in vehicle-treated adipocytes. Data expressed as mean ± SEM (n = 4). (C) Rosiglitazone induces OLR1 protein expression in 3T3-L1 adipocytes according to Western blot analysis. Histone deacetylase 2 (HDAC2) protein levels show equal loading. (D) Rosiglitazone (500 nM or 1 μM for 4 days) induces OLR1 mRNA expression in human adipocytes. Real-time PCR results expressed as mean ± SEM (n = 4). hOLR1, human OLR1. (E) Dose response for OLR1 induction by rosiglitazone and the non-TZD PPARγ ligand GW7845. (F) Induction of OLR1 by rosiglitazone (100 nM) is blocked by the PPARγ antagonist PD068235 (PD; 50 μM). rRNA, ribosomal RNA.