Fig. 1.

Affect of IL-6 deficiency on mononuclear cell infiltration in vivo. SES-induced peritoneal inflammation was established in WT and IL-6^-/- mice. (A) At defined time points, the peritoneal cavity was lavaged, and total lymphocyte (T and B cells) and monocytic cell numbers were determined by differential cell counting. (B) Infiltrating lymphocytes were phenotyped according to CD3⁺ or B220⁺ staining by flow cytometry. (C) Representative scatter plots for CD3⁺ or B220⁺ staining at 48 h are presented. Isotype antibody control staining is shown in Fig. 7, which is published as supporting information on the PNAS web site. Values represent the mean ± SEM (n = 10 mice per time point; *, P < 0.05).