Fig. 6.
Genetic modification of gp130-mediated STAT3 signals in vivo affects T cell recruitment after SES-induced inflammation. SES-induced peritoneal inflammation was established in the indicated strains, and at appropriate time intervals, the peritoneal cavity was lavaged and lymphocytes assessed by differential counting (A) and intraperitoneal CCL5 levels quantified by ELISA (B). Results expressed as mean ± SEM (n = 5 mice per group, *, P < 0.05). (C) Temporal changes in STAT1 and STAT3 phosphorylation were monitored by Western blot analysis of the peritoneal lining of WT, gp130Y757F/757F, gp130ΔSTAT/ΔSTAT, and gp130Y757F/Y757F:Stat3+/- mice after SES activation. Levels of extracellular signal-regulated kinase (ERK)-1/2 were monitored as an internal control, and densitometry of the banding intensity is presented as Fig. 8, which is published as supporting information on the PNAS web site.