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Tnfrh1 mRNA is restricted to the deep layers of the placenta. Two placentas from 12.5 dpc conceptuses were frozen and divided into superficial (fetal) and deep (maternal; mat) halves prior to RNA extraction. The Ipl cDNA probe is a specific marker for the labyrinthine trophoblast and therefore hybridizes to the lanes from the superficial halves of the placentas.
