LEF dependencies of antenna regulation and light-driven proton flux across the thylakoid membrane. Chlorophyll a fluorescence yield and ECS analyses were used to obtain estimates of energy-dependent exciton quenching (qE)(A) and steady-state proton flux into the lumen (νH+) (B), respectively, from 26 to 216 μmol photons m-2s-1 on leaves from A. thaliana Wt under ambient (372 ppm CO2/21% O2) (○) and low CO2 (LC; 50 ppm CO2/21% O2) (▵) air, as well as pgr5 under ambient air (▪) and plotted as a function of estimated LEF (18). Linear regressions of LEF versus νH+ are shown in B, the regression slopes of which are 2.035 (solid line), 2.038 (dotted line), and 1.774 (dashed line) for Wt ambient air, Wt/LC air, and pgr5 ambient air, respectively. Slopes for Wt/atmospheric and pgr5/atmospheric were judged by analysis of covariance to be statistically different (P < 0.05). Error bars represent SE for n = 3–6.