Figure 4:

Representative flow cytometry plots with associated summary bar graphs showing percentage of CD4+ and CD8+ T cells expressing A) IFN-γ, (B) TNF-α and (C) Gzmb in Lymph nodes and Spleens of MOC2 and MOC1 injected tumor bearing mice treated with vehicle control or CPSI-1306. D) Representative immunofluorescence staining of primary tumor tissue from vehicle control and CPSI-1306 treated MOC2 tumor bearing mice stained with DAPI (blue), CD8 (red), and Gzmb (green). Composite image is also shown to indicate CD8 cells expressing Gzmb. Bar graph depicting number of Gzmb producing CD8+ cells in tumors of vehicle control or CPSI-1306 treated mice. Quantification of graphical images were taken from four fields with N = 5 animals per group. Data are presented as mean ± SE. ^**P value < 0.01, * P value < 0.05.