Figure 5:

(A-E): Histogram plots of A) human oral epithelial cell line TE1177, B) human cancer cell lines SCC83, C) CA83, D) CAL27, and E) mouse oral cancer cell line MOC2, stained with MIF antibody or IgG isotype control antibody, and analyzed by flow cytometry. (F-J): Western blot images and graphical quantification of pERK, pAKT, and total ERK protein expression in (F) TE1177 normal oral epithelial cell line, (G) SCC83, (H) CA83, (I) CAL 27 and (J) MOC2 HNSCC cell lines treated with vehicle or CPSI-1306 for 24 hours at 1 and 10 μM. Western blot images were quantified with Image J, with proteins were normalized against GAPDH. * p value < 0.05; **p value < 0.01; ***p value < 0.001