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. 2025 Jan 10;16:584. doi: 10.1038/s41467-025-55936-5

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© The Author(s) 2025

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — FACS analyses. A Stimulation of tuft cells intratracheally with 100 µM clozapine-N-oxide (CNO) in Trpm5-DREADD mice (n = 5) led to a significant increase in neutrophil numbers within bronchoalveolar lavage fluid (BALF, Kurskal-Wallis test) in (B) trachea (One-way ANOVA) but not in (C) blood (One-way ANOVA) after 30 min, compared to untreated Trpm5-DREADD control (n = 6), Trpm5-DREADD sham treated (n = 4) and CNO-treated Trpm5^+/+ mice (n = 5). D In Trpm5-DREADD mice treated with 100 µM CNO (n = 7), the numbers of monocytes were significantly increased in BAL samples after 30 min compared to CNO-treated Trpm5^+/+mice (n = 7). sham-treated: n = 3, control=untreated: n = 6. Kruskal-Wallis test. E Interstitial macrophage (IM) numbers increased in BAL samples of Trpm5-DREADD mice treated with 100 µM CNO (n = 7) compared to CNO-treated Trpm5^+/+mice (n = 7). sham-treated: n = 5, control=untreated: n = 6. One-way ANOVA. F The number of alveolar macrophages (AM) remained unchanged in BAL-samples of Trpm5-DREADD mice treated with 100 µM CNO (n = 7). Trpm5^+/+: n = 7, sham-treated: n = 5, control=untreated: n = 6. Kruskal-Wallis test. G The dendritic cell (DC) number was significantly increased in BALF samples of Trpm5-DREADD mice (n = 6) treated with 100 µM CNO after 30 min compared to CNO-treated Trpm5^+/+ mice (n = 7). sham-treated: n = 4, control=untreated: n = 6. One-way ANOVA. H In lungs of Trpm5-DREADD mice (n = 7) treated with 100 µM CNO the DC number was significantly increased after 30 min compared to CNO-treated Trpm5^+/+ mice (n = 6). sham-treated: n = 5, control=untreated: n = 3. One-way ANOVA. A–H Source data are provided in the Source Data file. Data in panels A–H are presented as mean ± SEM.