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. 2025 Jan 10;16:584. doi: 10.1038/s41467-025-55936-5

Fig. 4. FACS analyses of dendritic cell (DC) numbers three days after tuft cell stimulation by denatonium (1 mM) or infection with Pseudomonas aeruginosa NH57388A.

Fig. 4

A DC (CD11b+, CD11c+, F4/80-) numbers in denatonium-treated or control tracheae of Trpm5+/+ (control n = 5, den n = 13) Trpm5−/− mice (control n = 5, den n = 7) and Chatfl/fl:Trpm5cre mice (control n = 4, den n = 7) after three days. Kruskal-Wallis test. B DC numbers in tracheae of control or denatonium-treated Panx1−/− (control n = 6, den n = 5) and Panx1+/+ mice (control n = 6, den n = 6). One-way ANOVA. C DC numbers three days post infection or in uninfected controls in Trpm5+/+ (control n = 7, P.a. n = 4), Trpm5−/− (control n = 6, P.a. n = 3) and Panx1−/− mice (control n = 6, P.a. n = 7). One-way ANOVA. D DC numbers in lungs of denatonium-treated (1 mM) and control Trpm5+/+ (control n = 5, den n = 12), Trpm5−/− mice (control n = 6, den n = 15) and Chatfl/fl:Trpm5cre mice (control n = 5, den n = 7). Kruskal-Wallis test. E DC numbers in lungs of denatonium-treated and control Panx1−/− (control n = 4, den n = 5) or Panx1+/+ mice (control n = 5, den n = 5). One-way ANOVA. F DC numbers in lungs of infected Trpm5+/+ (n = 4), Trpm5−/− (n = 3) and Panx1−/− mice (n = 10) and uninfected controls (Trpm5+/+ n = 6, Trpm5−/− n = 9, Panx1−/− n = 4). One-way ANOVA. G DC numbers in airway lymph nodes of denatonium-treated Trpm5+/+ (n = 10), control (n = 10) and Trpm5−/− mice (control n = 10, den n = 9). One-way ANOVA. H DC numbers in airway lymph nodes from denatonium-treated or control Panx1+/+ (n = 5) and Panx1−/− mice (n = 5). Kruskal-Wallis test. I Survival rates of Trpm5+/+ (n = 10), Trpm5−/− (n = 26) and Panx1−/− (n = 11) mice revealed decreased survival in Panx1−/− and Trpm5−/− mice compared to Trpm5+/+mice within the first 72 h after infection with P. aeruginosa NH57388A. Source data are provided in the Source Data file. Data in panels A–H are presented as mean ± SEM.