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. 2024 Dec 20;6(51):1375–1380. doi: 10.46234/ccdcw2024.273

Table 2. qPCR, enrichment, isolation, MALDI-TOF MS, WGS, and mouse bioassay experiments of samples collected in this study — Weihai City, China, July 2024.

Source qPCR before enrichment Enrichment in cooked meat medium and TPGYT medium qPCR after enrichment Colony morphology on the blood agar MALDI-TOF MS isolated strain WGS Mouse bioassay experiments
Abbreviation: qPCR=quantitative polymerasechain reaction; EV=environmental smear sample; ND=not detected; NEG=negative.
Patient A (feces) NEG Digestion of meat particles BoNT/A Flat, spreading growth pattern C. botulinum WHPT1 BoNT/A3 BoNT/A
Patient C (feces) BoNT/A Digestion of meat particles BoNT/A Flat, spreading growth pattern C. botulinum WHPT2 BoNT/A3 BoNT/A
Patient D (feces) NEG Digestion of meat particles BoNT/A Flat, spreading growth pattern C. botulinum WHPT3 BoNT/A3 BoNT/A
Patient E (feces) NEG Digestion of meat particles NEG ND ND ND ND ND
Asymptomatic Patient F (Anal swab) NEG Digestion of meat particles NEG ND ND ND ND ND
Asymptomatic Patient G (Anal swab) NEG Digestion of meat particles BoNT/A Flat, spreading growth pattern C. botulinum WHPT4 BoNT/A3 BoNT/A
Asymptomatic Patient H (Anal swab) NEG Digestion of meat particles NEG ND ND ND ND ND
pickled eggs BoNT/A Digestion of meat particles BoNT/A Flat, spreading growth pattern C. botulinum WHFD1 BoNT/A3 BoNT/A
EV 1 (raw eggs) ND Digestion of meat particles BoNT/A Flat, spreading growth pattern C. botulinum WHEV1 BoNT/A3 BoNT/A
EV 2 (Inner pot of rice cooker) ND No digestion of meat particles NEG ND ND ND ND ND
EV 3 (refrigerator) ND Digestion of meat particles BoNT/A Flat, spreading growth pattern C. botulinum WHEV2 BoNT/A3 ND
EV 4 (Sink) ND No digestion of meat particles NEG ND ND ND ND ND
EV 5 (pot) ND Digestion of meat particles BoNT/A Flat, spreading growth pattern C. botulinum WHEV3 BoNT/A3 ND
EV6 (kitchen cupboards) ND No digestion of meat particles NEG ND ND ND ND ND
EV 7 (Salt jar) ND Digestion of meat particles BoNT/A Flat, spreading growth pattern C. botulinum WHEV4 BoNT/A3 ND
EV 8 (casserole) ND Digestion of meat particles BoNT/A Flat, spreading growth pattern C. botulinum WHEV5 BoNT/A3 ND
EV 9 (Dining plate) ND Digestion of meat particles BoNT/A Flat, spreading growth pattern C. botulinum WHEV6 BoNT/A3 ND
EV 10 (garbage bin) ND Digestion of meat particles BoNT/A Flat, spreading growth pattern C. botulinum WHEV7 BoNT/A3 BoNT/A