Figure 2.
SAGA and ATAC HAT modules directly interact with VP16 AD. (A) Input extracts (0.2 μl) containing non-infected SF9 cell extract (control, lane 2) and SF9 cell extracts expressing the subunits of either SAGAHAT or ATACHAT (lanes 3 and 4). Fifty microliters of input extracts were then incubated with 50 μl of either GST–VP16 (B) or GST (C) beads, extensively washed and eluted. Ten percent of the total elutions were resolved by SDS–PAGE and analyzed by western blot with the indicated antibodies. Equal loading was tested by Coomassie brilliant blue staining of either GST–VP16 (B) or GST (C) protein containing beads. Each binding measurement was repeated in triplicate.