Figure 4.
Gal4–VP16 influences the acetylation of both the ATAC and SAGA HAT modules. Acetylation-coupled western blot assay of the ATACHAT (A) and SAGAHAT (B) modules at increasing concentrations with and without Gal4–VP16 at the indicated PTM sites. The fluorescence of Cy5-labeled histone H2A within the nucleosome was used to quantify nucleosome loading. Acetylation efficiency was tested by western blot using antibodies recognizing either histone H3K9ac or H4K5ac. Images are representative of three independent experiments with similar results (n = 3). Two-tailed, unpaired t-test with Welch’s correction was conducted using Prism 9 software. Error bars represent standard error of the mean. (C, D) Bar plots of panel (A). (E, F) Bar plots of panel (B).