Figure 7.

H₂S donors JZ-6 mitigates cellular lipotoxicity. (A) JZ-6 attenuated the ROS accumulation induced by free fatty acids (FFA). Representative fluorescent images of ROS and quantitative analysis of DCFH-DA fluorescent probe. Fluorescence intensity depicted as a ratio compared to the control group; Scale bars = 100 μm. (B) Lipid peroxidation levels (MDA levels) of steatosis HepG2 cells pretreated with NaHS (500 μmol/L), JZ-6 (20, 100, and 500 μmol/L), JZ-8 (20, 100, and 500 μmol/L) and JZ-9 (500 μmol/L). (C) Inhibition of ferroptosis induced by RSL3 (4 μmol/L) in HepG2 cells. (D) Reduction of lysosomal MDA levels. (E) Determination of lysosomal membrane permeabilization in HepG2 cells with acridine orange staining. Scale bars = 20 μm. (F) Schematic diagram showing multidimensional protection against lipotoxicity of JZ-6. Data represent mean ± SD of independent experiments (n = 6). ∗∗∗∗P < 0.0001, ∗∗∗P < 0.001, ∗∗P < 0.01 and ∗P < 0.05 versus model group and ^####P < 0.0001 versus control group. ns, not significant.