Figure 5.

The synergistic regulation of healthy and damaged mitochondria via mNP-Mito in vivo. (A) Uptake of MitoTracker Red-marked mNP-Mito in retinal ganglionic cell layer of normal mice (RGL: retinal ganglion layer; IPL: inner plexiform layer; INL: inner nuclear layer; OPL: outer plexiform layer; ONL: outer nuclear layer) (24 h); (B) The distribution of MitoTracker Green-labeled Cy3-mNP-Mito in vivo (24 h), white arrowheads represent the merged spots of exogenous mitochondria and Cy3-mRNA in RGL; (C, D) PARKIN protein expression in the whole cells according to immunofluorescence staining (C) and Western blot (D) assays in Rot-induced mice, dashed line area: RGL; (E) Quantitative analysis of PARKIN expression using ImageJ software based on the data presented in (D); (F) Mitochondrial PARKIN protein expression and ubiquitination of mitochondrial protein in Rot-induced mice in various treatment groups. Data are shown as the mean ± SD, n = 3 independent samples per group in (E). Statistical significance was shown by one-way ANOVA with Tukey's honest significant difference (HSD) post hoc test (E).