Table 2:

Overview of the preanalytical Conditions for Circulating miRNAs use in diagnostic.

S.No.	Category	Description	References
1.	Sample Types	Blood samples: EDTA, heparin, or citrate tubes.	[37]
2.	Tube Recommendations	RNase-free tubes and collection devices are essential. Tubes designed for plasma or serum collection are recommended.	[38,39]
3.	Isolation Methodologies	Column-based Kits: miRNeasy Mini Kit (Qiagen), NucleoSpin miRNA Kit (Macherey-Nagel).	[44]
		Magnetic Bead-based Kits: Exosome Plus™ MicroRNA Isolation Kit (Thermo Fisher Scientific).	[45]
		Phenol-Chloroform Extraction: The traditional method requires careful handling. (Invitrogen TM TRIzolTM Reagent and QIAzol Lysis Reagent).	[46]
4.	Small RNA Library Prep Kits for NGS	TruSeq Small RNA Library Prep Kit (Illumina): Streamlined workflow for small RNA sequencing.	[48]
		NEBNext Small RNA Library Prep Kit (New England Biolabs): High sensitivity and accuracy for small RNA capture.	[49]
5.	Considerations	Ensure compatibility between the library preparation kit and the RNA isolation method to maintain high-quality sequencing results.	[50]