Table 3:

Emerging techniques with their key features outcome and significance.

Technique	Key Features	Outcome and Significance	References
Next Generation Sequencing	Comprehensive profiling of miRNAs	NGS enables the identification of dysregulated miRNAs, offering potential non-invasive biomarkers for early lung cancer detection.	[73]
Digital PCR	Absolute quantification of miRNAs	dPCR-based miRNA profiling holds potential for breast cancer diagnosis and personalized medicine approaches.	[100]
Single Cell Sequencing	Analysis of miRNA expression at the single-cell level	Single-cell miRNA profiling provides insights into miRNA expression heterogeneity during neuronal development.	[101]
Nanostring nCounter Technology	Simultaneous detection and quantification of multiple miRNAs in a single assay	Nanostring nCounter technology enables the identification of miRNA biomarkers for bladder cancer diagnosis and prognosis.	[102]
Droplet Digital PCR	Absolute quantification of miRNAs with high precision and sensitivity	Identified a miRNA-based signature for ddPCR-based miRNA profiling shows potential as a non-invasive diagnostic tool for colorectal cancer.	[103]
Microarrays	Simultaneous detection and quantification of multiple miRNAs	Microarray profiling identified miRNAs involved in myocardial infarction, providing insights into cardiac disease mechanisms.	[104]
Small RNAseq	Sequencing of small RNA molecules, including miRNAs	Small RNA-Seq enables the identification of miRNAs associated with pregnancy-related complications.	[105]
qRTPCR	Widely used technique for miRNA expression analysis	qRTPCR-based miRNA profiling provides insights into the involvement of dysregulated miRNAs in Alzheimer’s disease	[106]
LNA-based miRNA profiling.	Enhanced probe binding affinity using Locked Nucleic Acid (LNA) technology	LNA-based miRNA profiling allows for the identification of region-specific miRNA expression patterns in the bra in.	[107]
Bead-based miRNA profiling	Quantitative analysis of miRNA expression levels using bead arrays	Bead-based miRNA profiling enables the identification of miRNA signatures with potential clinical implications in hepatocellular carcinoma.	[108]
Mass Spectrometry	Mass spectrometry-based methods for miRNA profiling	Mass spectrometry-based miRNA profiling offers a potential avenue for identifying miRNA biomarkers in prostate cancer.	[109]
Functional miRNA profiling	Study of miRNA activity and their effects on target genes	Functional miRNA profiling provides insights into the regulatory functions of specific miRNAs in gene expression and cancer biology.	[110]