Abstract
Phospholipid depletion substantially inhibited the maximum demonstrable activities of the forward (glucuronidation) and reverse reactions of UDP-glucuronyltransferase towards p-nitrophenol in guinea-pig liver microsomal preparations. Dispersions of liver phospholipids restored activity, whereas non-phospholipid amphipaths failed to do so effectively. These results suggest that the system is probably phospholipid-dependent rather than conformationally constrained by phospholipids.
Full text
PDFSelected References
These references are in PubMed. This may not be the complete list of references from this article.
- Attwood D., Graham A. B., Wood G. C. The phospholipid-dependence of uridine diphosphate glucuronyltransferase. Reactivation of phospholipase A-inactivated enzyme by phospholipids and detergents. Biochem J. 1971 Aug;123(5):875–882. doi: 10.1042/bj1230875. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Berry C., Stellon A., Hallinan T. Guinea pig liver microsomal UDP-glucuronyltransferase: compartmented or phospholipid-constrained? Biochim Biophys Acta. 1975 Oct 22;403(2):335–344. doi: 10.1016/0005-2744(75)90063-7. [DOI] [PubMed] [Google Scholar]
- Cater B. R., Trivedi P., Hallinan T. Inhibition of glucose 6-phosphatase by pure and impure C-type phospholipases. Reactivation by phospholipid dispersions and protection by serum albumin. Biochem J. 1975 May;148(2):279–294. doi: 10.1042/bj1480279. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Coleman R., Bramley T. A. Hydrolysis of erythrocyte membrane phospholipids by a preparation of phospholipase C from Clostridium Welchii. Deactivation of (Ca-2+, Mg-2+)-ATPase and its reactivation by added lipids. Biochim Biophys Acta. 1975 Apr 8;382(4):565–575. doi: 10.1016/0005-2736(75)90223-0. [DOI] [PubMed] [Google Scholar]
- Graham A. B., Wood G. C. The phospholipid-dependence of UDP-glucuronyltransferase. Biochem Biophys Res Commun. 1969 Nov 6;37(4):567–575. doi: 10.1016/0006-291x(69)90846-8. [DOI] [PubMed] [Google Scholar]
- Hänninen O., Puukka R. Activation of microsomal UDP glucuronyltransferase by phospholipases. Chem Biol Interact. 1971 Aug;3(4):282–284. doi: 10.1016/0009-2797(71)90061-5. [DOI] [PubMed] [Google Scholar]
- Jansen P. L., Arias I. M. Delipidation and reactivation of UDPglucuronosyltransferase from rat liver. Biochim Biophys Acta. 1975 May 23;391(1):23–38. [PubMed] [Google Scholar]
- Kuriyama Y. Studies on microsomal nucleoside diphosphatase of rat hepatocytes. Its purification, intramembranous localization, and turnover. J Biol Chem. 1972 May 25;247(10):2979–2988. [PubMed] [Google Scholar]
- Lueders K. K., Kuff E. L. Spontaneous and detergent activation of a glucuronyltransferase in vitro. Arch Biochem Biophys. 1967 Apr;120(1):198–203. doi: 10.1016/0003-9861(67)90614-5. [DOI] [PubMed] [Google Scholar]
- Rao G. S., Rao M. L., Breuer H. Studies on a solubilized oestriol 16 -glucuronyltransferase from human liver microsomes. J Steroid Biochem. 1972 Jan;3(1):1–10. doi: 10.1016/0022-4731(72)90005-2. [DOI] [PubMed] [Google Scholar]
- Vessey D. A., Zakim D. Regulation of microsomal enzymes by phospholipids. II. Acitvation of hepatic uridine diphosphate-glucuronyltransferase. J Biol Chem. 1971 Aug 10;246(15):4649–4656. [PubMed] [Google Scholar]
- Vessey D. A., Zakim D. Regulation of microsomal enzymes by phospholipids. IV. Species differences in the properties of microsomal UDP-glucuronyltransferase. Biochim Biophys Acta. 1972 Apr 7;268(1):61–69. doi: 10.1016/0005-2744(72)90197-0. [DOI] [PubMed] [Google Scholar]
- Winsnes A. Kinetic properties of different forms of hepatic UDPglucuronyltransferase. Biochim Biophys Acta. 1972 Oct 12;284(2):394–405. doi: 10.1016/0005-2744(72)90135-0. [DOI] [PubMed] [Google Scholar]
- Zakim D., Goldenberg J., Vessey D. A. Influence of membrane lipids on the regulatory properties of UDP-glucuronyltransferase. Eur J Biochem. 1973 Sep 21;38(1):59–63. doi: 10.1111/j.1432-1033.1973.tb03033.x. [DOI] [PubMed] [Google Scholar]
- Zakim K., Vessey D. A. Regulation of microsomal enzymes by phospholipids. IX. Production of uniquely modified forms of microsomal UDP-glucuronyltransferase by treatment with phospholipase A and detergents. Biochim Biophys Acta. 1975 Nov 20;410(1):61–73. doi: 10.1016/0005-2744(75)90207-7. [DOI] [PubMed] [Google Scholar]