Skip to main content
PMC home page
Biochemical Journal logoLink to Biochemical Journal
. 1976 Mar 15;154(3):783–785. doi: 10.1042/bj1540783

Effect on uridine diphosphate glucuronyltransferase activity of depleting and restoring phospholipids to guinea-pig liver microsomal preparations.

C S Berry, M Caldecourt, T Hallinan
PMCID: PMC1172782  PMID: 821474

Abstract

Phospholipid depletion substantially inhibited the maximum demonstrable activities of the forward (glucuronidation) and reverse reactions of UDP-glucuronyltransferase towards p-nitrophenol in guinea-pig liver microsomal preparations. Dispersions of liver phospholipids restored activity, whereas non-phospholipid amphipaths failed to do so effectively. These results suggest that the system is probably phospholipid-dependent rather than conformationally constrained by phospholipids.

Full text

PDF
783

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Attwood D., Graham A. B., Wood G. C. The phospholipid-dependence of uridine diphosphate glucuronyltransferase. Reactivation of phospholipase A-inactivated enzyme by phospholipids and detergents. Biochem J. 1971 Aug;123(5):875–882. doi: 10.1042/bj1230875. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berry C., Stellon A., Hallinan T. Guinea pig liver microsomal UDP-glucuronyltransferase: compartmented or phospholipid-constrained? Biochim Biophys Acta. 1975 Oct 22;403(2):335–344. doi: 10.1016/0005-2744(75)90063-7. [DOI] [PubMed] [Google Scholar]
  3. Cater B. R., Trivedi P., Hallinan T. Inhibition of glucose 6-phosphatase by pure and impure C-type phospholipases. Reactivation by phospholipid dispersions and protection by serum albumin. Biochem J. 1975 May;148(2):279–294. doi: 10.1042/bj1480279. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Coleman R., Bramley T. A. Hydrolysis of erythrocyte membrane phospholipids by a preparation of phospholipase C from Clostridium Welchii. Deactivation of (Ca-2+, Mg-2+)-ATPase and its reactivation by added lipids. Biochim Biophys Acta. 1975 Apr 8;382(4):565–575. doi: 10.1016/0005-2736(75)90223-0. [DOI] [PubMed] [Google Scholar]
  5. Graham A. B., Wood G. C. The phospholipid-dependence of UDP-glucuronyltransferase. Biochem Biophys Res Commun. 1969 Nov 6;37(4):567–575. doi: 10.1016/0006-291x(69)90846-8. [DOI] [PubMed] [Google Scholar]
  6. Hänninen O., Puukka R. Activation of microsomal UDP glucuronyltransferase by phospholipases. Chem Biol Interact. 1971 Aug;3(4):282–284. doi: 10.1016/0009-2797(71)90061-5. [DOI] [PubMed] [Google Scholar]
  7. Jansen P. L., Arias I. M. Delipidation and reactivation of UDPglucuronosyltransferase from rat liver. Biochim Biophys Acta. 1975 May 23;391(1):23–38. [PubMed] [Google Scholar]
  8. Kuriyama Y. Studies on microsomal nucleoside diphosphatase of rat hepatocytes. Its purification, intramembranous localization, and turnover. J Biol Chem. 1972 May 25;247(10):2979–2988. [PubMed] [Google Scholar]
  9. Lueders K. K., Kuff E. L. Spontaneous and detergent activation of a glucuronyltransferase in vitro. Arch Biochem Biophys. 1967 Apr;120(1):198–203. doi: 10.1016/0003-9861(67)90614-5. [DOI] [PubMed] [Google Scholar]
  10. Rao G. S., Rao M. L., Breuer H. Studies on a solubilized oestriol 16 -glucuronyltransferase from human liver microsomes. J Steroid Biochem. 1972 Jan;3(1):1–10. doi: 10.1016/0022-4731(72)90005-2. [DOI] [PubMed] [Google Scholar]
  11. Vessey D. A., Zakim D. Regulation of microsomal enzymes by phospholipids. II. Acitvation of hepatic uridine diphosphate-glucuronyltransferase. J Biol Chem. 1971 Aug 10;246(15):4649–4656. [PubMed] [Google Scholar]
  12. Vessey D. A., Zakim D. Regulation of microsomal enzymes by phospholipids. IV. Species differences in the properties of microsomal UDP-glucuronyltransferase. Biochim Biophys Acta. 1972 Apr 7;268(1):61–69. doi: 10.1016/0005-2744(72)90197-0. [DOI] [PubMed] [Google Scholar]
  13. Winsnes A. Kinetic properties of different forms of hepatic UDPglucuronyltransferase. Biochim Biophys Acta. 1972 Oct 12;284(2):394–405. doi: 10.1016/0005-2744(72)90135-0. [DOI] [PubMed] [Google Scholar]
  14. Zakim D., Goldenberg J., Vessey D. A. Influence of membrane lipids on the regulatory properties of UDP-glucuronyltransferase. Eur J Biochem. 1973 Sep 21;38(1):59–63. doi: 10.1111/j.1432-1033.1973.tb03033.x. [DOI] [PubMed] [Google Scholar]
  15. Zakim K., Vessey D. A. Regulation of microsomal enzymes by phospholipids. IX. Production of uniquely modified forms of microsomal UDP-glucuronyltransferase by treatment with phospholipase A and detergents. Biochim Biophys Acta. 1975 Nov 20;410(1):61–73. doi: 10.1016/0005-2744(75)90207-7. [DOI] [PubMed] [Google Scholar]

Articles from Biochemical Journal are provided here courtesy of The Biochemical Society

RESOURCES