Figure 6.
Cytoplasmic CyKILRb is a pro-survival factor
The depicted cell lines were transfected with scrambled control (SCR) or CyKILRb siRNA (siCyKILRb). After 48 h, total RNA was extracted or the cells were assessed for the indicated biological mechanisms with the 48 h after transfection acting as the 0 time point (A–D). In addition, 48-h post-transfected H2009 and H1792 cells were subsequently mixed with Matrigel in a 1:1 ratio and injected sc into the flanks of 5-week-old female NOD-SCID mice (1 × 106 cells per mouse per flank). The left flank received Scramble-treated cells, while the right flank received knockdown cells (E). (A) RNA levels of CyKILRb siRNA-treated samples compared with scrambled siRNA control (SCR) samples normalized to GAPDH mRNA. −RT controls were included in all experiments and replicates with no signal observed. (B) Proliferation of CyKILRb siRNA-treated cells compared with scrambled siRNA control cells at the 48-h time point. (C) Migration of CyKILRb siRNA-treated cells compared with scrambled siRNA control cells at the 24-h time point. (D) Plate clonogenic survival of CyKILRb siRNA-treated cells compared with scrambled siRNA control cells. (E) Tumor volume of siRNA-scramble control or CyKILRb siRNA treated H2009 or H1792. The data are presented as boxplots (n ≥ 3 replicates), and statistical analysis was performed using the unpaired t test with Welch’s correction.