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. 1976 Jun 1;155(3):543–548. doi: 10.1042/bj1550543

Study of poly-L-lysine conformations in aqueous methanol solution by using polarized Raman techniques.

I W Shepherd
PMCID: PMC1172876  PMID: 949317

Abstract

Raman polarization measurements of the amide I band are reported in ionized poly-L-lysine dissolved in aqueous methanol. The observed changes with methanol concentration, attributed to changes in coil conformation and to the helix-coil transition, represent a novel method of measuring polymer conformation. Polarization measurements as a function of temperature yield values of the energy differences between rotational isomeric states in the coil. deltaH, of 8.8 +/- 0.7, 10.4 +/- 1.1 and 10.8 +/- 1.5 kJ/mol at methanol concentrations (v/v) of 85, 80 and 70% respectively. The stabilization energy of the helix is estimated at 9.3 kJ/mol.

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Selected References

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