Figure 1.

Triplex formation by 23mer TFOs on 23 bp c-myc P2 sequence. The pyrimidine-rich strand of the target duplex was labeled with ³²P and annealed to the complementary purine-rich strand. Indicated concentrations of TFOs Myc CT or Myc GTC were incubated with duplex (2 nM) overnight at 37°C and pH 5.6 or 7.2, except Myc CT at pH 7.2, where incubation was at 4°C. Complexes were resolved on non-denaturing gels at the same pH and at 20°C. T, triplex DNA; D, duplex DNA.