Figure 4.

TFO-mediated inhibition of nuclear protein binding to myc P2 sequence. Myc P2 probe was pre-incubated alone (0) or with indicated concentrations of TFOs and control oligos in HEPES–MgCl₂ for 2 h at 37°C. MCF-7 nuclear extracts were added, mixtures incubated, and complexes resolved on a polyacrylamide gel as described in Figure 3. Sp1 and Sp3 complexes are indicated. U, unbound DNA probe.